Abstract Single-molecule localization microscopy requires sparse activation of emitters to circumvent the diffraction limit. In densely labeled or thick samples. overlap of emitter images is inevitable. Single-molecule localization of these samples results in a biased parameter estimate with a wrong model of the number of emitters. https://www.nacrack.com/great-pick-Refurbished-Sawgrass-Virtuoso-SG500-Complete-Sublijet-Sublimation-Printer-Kit-flash-buy/