The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR solutions had been digested and cloned into a pET21a+ vector using NdeI and XhoI restriction enzymes. A degree mutation was introduced by the short transform https://d-326370122.suomiblog.com/the-definitive-guide-to-arv-471-42021023